Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup.
Identifieur interne : 000389 ( Main/Exploration ); précédent : 000388; suivant : 000390Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup.
Auteurs : Marie-Francoise Noirot-Gros [États-Unis] ; Shalaka V. Shinde [États-Unis] ; Chase Akins [États-Unis] ; Jessica L. Johnson [États-Unis] ; Sarah Zerbs [États-Unis] ; Rosemarie Wilton [États-Unis] ; Kenneth M. Kemner [États-Unis] ; Philippe Noirot [États-Unis] ; Gyorgy Babnigg [États-Unis]Source :
- Frontiers in plant science [ 1664-462X ] ; 2020.
Abstract
Coupling microfluidics with microscopy has emerged as a powerful approach to study at cellular resolution the dynamics in plant physiology and root-microbe interactions (RMIs). Most devices have been designed to study the model plant Arabidopsis thaliana at higher throughput than conventional methods. However, there is a need for microfluidic devices which enable in vivo studies of root development and RMIs in woody plants. Here, we developed the RMI-chip, a simple microfluidic setup in which Populus tremuloides (aspen tree) seedlings can grow for over a month, allowing continuous microscopic observation of interactions between live roots and rhizobacteria. We find that the colonization of growing aspen roots by Pseudomonas fluorescens in the RMI-chip involves dynamic biofilm formation and dispersal, in keeping with previous observations in a different experimental set-up. Also, we find that whole-cell biosensors based on the rhizobacterium Bacillus subtilis can be used to monitor compositional changes in the rhizosphere but that the application of these biosensors is limited by their efficiency at colonizing aspen roots and persisting. These results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacterial root colonizer.
DOI: 10.3389/fpls.2020.00408
PubMed: 32351525
PubMed Central: PMC7174594
Affiliations:
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Kemner</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Argonne National Laboratory, Lemont, IL</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Noirot, Philippe" sort="Noirot, Philippe" uniqKey="Noirot P" first="Philippe" last="Noirot">Philippe Noirot</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Argonne National Laboratory, Lemont, IL</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Babnigg, Gyorgy" sort="Babnigg, Gyorgy" uniqKey="Babnigg G" first="Gyorgy" last="Babnigg">Gyorgy Babnigg</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Argonne National Laboratory, Lemont, IL</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coupling microfluidics with microscopy has emerged as a powerful approach to study at cellular resolution the dynamics in plant physiology and root-microbe interactions (RMIs). Most devices have been designed to study the model plant <i>Arabidopsis thaliana</i> at higher throughput than conventional methods. However, there is a need for microfluidic devices which enable <i>in vivo</i> studies of root development and RMIs in woody plants. Here, we developed the RMI-chip, a simple microfluidic setup in which <i>Populus tremuloides</i> (aspen tree) seedlings can grow for over a month, allowing continuous microscopic observation of interactions between live roots and rhizobacteria. We find that the colonization of growing aspen roots by <i>Pseudomonas fluorescens</i> in the RMI-chip involves dynamic biofilm formation and dispersal, in keeping with previous observations in a different experimental set-up. Also, we find that whole-cell biosensors based on the rhizobacterium <i>Bacillus subtilis</i> can be used to monitor compositional changes in the rhizosphere but that the application of these biosensors is limited by their efficiency at colonizing aspen roots and persisting. These results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacterial root colonizer.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32351525</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Functional Imaging of Microbial Interactions With Tree Roots Using a Microfluidics Setup.</ArticleTitle><Pagination><MedlinePgn>408</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2020.00408</ELocationID><Abstract><AbstractText>Coupling microfluidics with microscopy has emerged as a powerful approach to study at cellular resolution the dynamics in plant physiology and root-microbe interactions (RMIs). Most devices have been designed to study the model plant <i>Arabidopsis thaliana</i> at higher throughput than conventional methods. However, there is a need for microfluidic devices which enable <i>in vivo</i> studies of root development and RMIs in woody plants. Here, we developed the RMI-chip, a simple microfluidic setup in which <i>Populus tremuloides</i> (aspen tree) seedlings can grow for over a month, allowing continuous microscopic observation of interactions between live roots and rhizobacteria. We find that the colonization of growing aspen roots by <i>Pseudomonas fluorescens</i> in the RMI-chip involves dynamic biofilm formation and dispersal, in keeping with previous observations in a different experimental set-up. Also, we find that whole-cell biosensors based on the rhizobacterium <i>Bacillus subtilis</i> can be used to monitor compositional changes in the rhizosphere but that the application of these biosensors is limited by their efficiency at colonizing aspen roots and persisting. These results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacterial root colonizer.</AbstractText><CopyrightInformation>Copyright © 2020 Noirot-Gros, Shinde, Akins, Johnson, Zerbs, Wilton, Kemner, Noirot and Babnigg.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Noirot-Gros</LastName><ForeName>Marie-Francoise</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shinde</LastName><ForeName>Shalaka V</ForeName><Initials>SV</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Akins</LastName><ForeName>Chase</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Johnson</LastName><ForeName>Jessica L</ForeName><Initials>JL</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zerbs</LastName><ForeName>Sarah</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wilton</LastName><ForeName>Rosemarie</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kemner</LastName><ForeName>Kenneth M</ForeName><Initials>KM</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Noirot</LastName><ForeName>Philippe</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Babnigg</LastName><ForeName>Gyorgy</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Biosciences Division, Argonne National Laboratory, Lemont, IL, United States.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacillus subtilis</Keyword><Keyword MajorTopicYN="N">Populus tremuloides</Keyword><Keyword MajorTopicYN="N">Pseudomonas fluorescens</Keyword><Keyword MajorTopicYN="N">biosensor</Keyword><Keyword MajorTopicYN="N">microfluidics</Keyword><Keyword MajorTopicYN="N">root-microbe interaction</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>20</Day></PubMedPubDate><PubMedPubDate 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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32351525" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |